🧪 Core Culture Equipment
- CO₂ Incubator (Essential for mammalian/stem cell growth)
- Laminar Flow Hood / Biosafety Cabinet (Class II) (Sterile work environment)
- Water-Jacketed Incubator (for more sensitive or long-term cultures)
- Cryostorage System (Liquid nitrogen tanks for cell line freezing and storage)
🔬 Microscopy & Imaging
- Inverted Phase-Contrast Microscope (Daily cell health checks)
- Fluorescence Microscope (for stem cell marker staining, GFP-tagged cells)
- Live-Cell Imaging System (for time-lapse videos of cell behavior, optional but powerful)
🧴 Consumables & Basic Tools
- Tissue Culture Flasks, Plates, and Dishes (T-25, T-75, 6-well, 24-well, 96-well plates)
- Cell Scrapers (for harvesting adherent cells)
- Sterile Pipette Tips (filter tips)
- Sterile Serological Pipettes (2, 5, 10, 25 mL)
- Cryovials (for freezing cells)
- Conical Tubes (15 mL and 50 mL)
- Pasteur Pipettes
💻 Analytical & Monitoring Equipment
- Automated Cell Counter (like Countess™ or hemocytometer manual counting)
- pH Meter (to check media pH occasionally)
- Spectrophotometer / Plate Reader (for proliferation assays, viability, ELISAs)
🔥 Sterilization & Decontamination
- Autoclave (for sterilizing glassware, solutions)
- 70% Ethanol Sprayers (for wiping surfaces)
- UV Light in Biosafety Cabinet (for surface sterilization)
- Mycoplasma Detection Kits (to regularly check for contamination)
🥼 Safety & PPE
- Lab Coats (preferably disposable)
- Sterile Gloves (powder-free)
- Face Masks
- Eye Protection / Goggles
- Biohazard Waste Bins
- Sharps Disposal Containers
🧫 Special Reagents and Culture Media
- Basal Media (DMEM, RPMI, MEM, depending on the cell type)
- Serum (Fetal Bovine Serum [FBS], or defined alternatives)
- Antibiotics (Penicillin-Streptomycin, Amphotericin B)
- Trypsin-EDTA (for passaging cells)
- Stem Cell Supplements (for stem cell work: bFGF, LIF, specialized growth factors)
- Feeder Layers or Coating Agents (e.g., Matrigel®, gelatin, laminin for stem cell adherence)
❄️ Cold Storage
- 4°C Refrigerator (media, reagents)
- -20°C Freezer (buffers, antibiotics, enzymes)
- -80°C Freezer (for short-term cell stock storage)
- Liquid Nitrogen Tank (for long-term cryopreservation)
📚 Documentation & Data
- Cell Culture Logbooks (track passages, splits, thaw/freeze events)
- SOPs (Standard Operating Procedures) (for training and compliance)
- Label Printers and Cryo-labels (for organized cell inventory)
✅ Special Stem Cell Culture Extras:
- Hypoxia chamber (for low-oxygen culturing, optional)
- ROCK Inhibitor (Y-27632) for iPSC survival during passaging
- Flow Cytometer (to analyze stem cell surface markers)